Making Preparations for Mitosis and Meiosis

When I wanted to study the form of chromosomes of a cell, then the right moment was when cells are dividing both mitosis and meiosis. Mitotic division occurs in cells that produce the same cells before and are diploid cells, while meiosis occurs in cell division that produces gametes which are haploid cells.

The process of making preparations for mitosis and meiosis in general there are two kinds, namely the method pejetan (squash) and the method of slices. While the chromosome is also necessary to see chromosome dye which include acetic orcein, aniline gentian violet, haematoxylin-Whitman, and so forth. Difficulty making preparations for mitosis and meiosis is to determine the appropriate time during cell division, this should be tested to determine the time of division. Here is a way of making preparations for mitosis and mitosis.

Making Preparations for Mitosis-Meiosis with Pejetan Method (Squash)

To make preparations for mitosis should use the root of onion or garlic, while making preparations meiosis using chilli flower buds or flower buds Lilium. The time is right to know the time of mitotic division is at the root of onion roots grow about 2 mm, while for meiotic flower buds chilli at night (around 22.00).

Ingredients: Farmer's solution: glacial acetic acid, absolute alcohol; haematoxylin whitmann; concentrated HCl; Hoyer's mounting; aquades.
Step:
Fixation: Do the fixation of specimens into the Farmer's solution, at least 1 hour and up to 24 hours.

Hydrolysis: After fixation, the specimens in hirolisis by using concentrated HCl and 95% alcohol with a ratio of 1:3 for 30 minutes. The purpose of the hydrolysis is the network for easy dipejet soften in the glass object.

Pencucuian: washing stage aims to remove any residual HCl in the network. Washing is done by using absolute alcohol three times each for approximately 7 minutes.

Staining: After being washed with alcohol, it is stained by using haematoxylin whitmann for 20 minutes. To maximize the absorption of color into the chromosome it is necessary to be mixed with rusty metal with the aim of increasing the oxidation reaction with the chromosome dye.

Washing: Washing second using glacial acetic acid. The excess tissue color will come out. At this stage determines the quality of the preparations mitosis meiosis.

Bonding: This phase specimens glued on glass objects using Hoyer's mounting dipejet way for cells to spread, then dried preparations.

 Onion root mitosis.

 Meiotic flower buds of chili.

Source : www.biology-community.blogspot.com